Journal: Biomedicines

Article Title: Idebenone Protects against Spontaneous Chronic Murine Colitis by Alleviating Endoplasmic Reticulum Stress and Inflammatory Response

doi: 10.3390/biomedicines8100384

Figure Lengend Snippet: Effect of idebenone on mRNA levels of endoplasmic reticulum stress (ER stress) markers in the Winnie model of spontaneous chronic colitis. Gene expression of ( A ) GRP78 , ( B ) CHOP , ( C ) ATF6 , ( D ) XBP-1 and ( E ) PERK were determined for the proximal (PC) and distal colon (DC) for all the three groups. mRNA levels were normalized to GAPDH gene and presented as fold change. Data represents mean ± SEM ( n = 4/group) using One-way ANOVA followed by Tukey’s post-test, where * p < 0.05, ** p < 0.01 and *** p < 0.001.

Article Snippet: The membranes were then blocked in 5% skim milk prepared in TBST and incubated overnight with primary antibody against NQO-1 (1:1000, ab34173, Abcam), GRP78 (1:1000, NBP1-06274, Novus biologicals) CHOP (1:1000, NBP2-13172, Novus Biologicals) and β-actin (1:8000, NB600-503, Novus Biologicals) at 4 °C.

Techniques: Gene Expression

Journal: Biomedicines

Article Title: Idebenone Protects against Spontaneous Chronic Murine Colitis by Alleviating Endoplasmic Reticulum Stress and Inflammatory Response

doi: 10.3390/biomedicines8100384

Figure Lengend Snippet: Effect of idebenone on endoplasmic reticulum stress (ER stress) and oxidative stress in spontaneous chronic colitis in Winnie mice. ( A ) protein levels of GRP78, CHOP and NQO-1 were analyzed using western blotting of tissue samples from the distal colon (DC), ( B – D ) densitometry of GRP78, CHOP and NQO-1 expression. Band densities were normalized to β-actin. ( E ) malondialdehyde (MDA) levels in distal colon. Data represents mean ± SEM ( n = 3/group). Statistical significance was determined using One-way ANOVA followed by Tukey’s post-test, where * p < 0.05, ** p < 0.01 and *** p < 0.001 and ns: nonsignificant.

Article Snippet: The membranes were then blocked in 5% skim milk prepared in TBST and incubated overnight with primary antibody against NQO-1 (1:1000, ab34173, Abcam), GRP78 (1:1000, NBP1-06274, Novus biologicals) CHOP (1:1000, NBP2-13172, Novus Biologicals) and β-actin (1:8000, NB600-503, Novus Biologicals) at 4 °C.

Techniques: Western Blot, Expressing